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Related Experiment Video

Updated: Dec 24, 2025

Rapid Mix Preparation of Bioinspired Nanoscale Hydroxyapatite for Biomedical Applications

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Published on: February 23, 2017

Multifunctional hydroxyapatite nanoparticle-based affinity adsorbent with sensing and fluorescence imaging capacity.

Shasha Yao¹, Yu Zhang, Junli Zhang

¹Key Laboratory for Special Functional Materials, Henan University, Kaifeng 475004, P. R. China. zhaoyb902@henu.edu.cn.

Journal of Materials Chemistry. B

|April 9, 2020

Summary

Synthesized novel nanoparticles (Ni(OH)₂/HAP-RBH) effectively purify His-tagged proteins and detect platinum ions (Pt²⁺) with high sensitivity. These versatile nanoparticles also show potential for living cell imaging.

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Area of Science:

Materials Science
Nanotechnology
Biochemistry

Background:

Developing novel nanomaterials for efficient protein purification and metal ion detection is crucial.
Nickel hydroxide/hydroxyapatite nanoparticles offer unique properties for biomedical applications.

Purpose of the Study:

To synthesize and characterize rhodamine B hydrazide-coated nickel hydroxide/hydroxyapatite nanoparticles (Ni(OH)₂/HAP-RBH).
To evaluate the efficacy of these nanoparticles as affinity adsorbents for histidine-tagged (His-tagged) proteins.
To assess the potential of Ni(OH)₂/HAP-RBH nanoparticles for selective and sensitive detection of platinum ions (Pt²⁺) and their application in cell imaging.

Main Methods:

Facile hydrothermal synthesis of Ni(OH)₂/HAP-COOH nanoparticles.
Conjugation of rhodamine B hydrazide (RBH) onto the nanoparticle surface.
Assessment of protein adsorption capacity and selectivity for His-tagged proteins.
Spectroscopic analysis for Pt²⁺ detection, including fluorescence and absorption changes.
Cytotoxicity evaluation and application in living cell imaging.

Main Results:

Successfully synthesized Ni(OH)₂/HAP-RBH nanoparticles with a high protein adsorption capacity (115 mg g^-1).
Demonstrated excellent selectivity and sensitive detection of Pt²⁺ with a detection limit of 0.03 μM, showing fluorescence enhancement at 578 nm.
Observed low cytotoxicity, indicating suitability for biological applications.
Confirmed the utility of these nanoparticles as fluorescent probes for Pt²⁺ and for living cell imaging.

Conclusions:

Ni(OH)₂/HAP-RBH nanoparticles are effective affinity adsorbents for His-tagged protein purification.
These nanoparticles serve as sensitive and selective fluorescent probes for Pt²⁺ detection.
The synthesized nanoparticles exhibit low cytotoxicity and hold promise for bioimaging applications.