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Updated: Dec 24, 2025

Rapid Mix Preparation of Bioinspired Nanoscale Hydroxyapatite for Biomedical Applications

Rapid Mix Preparation of Bioinspired Nanoscale Hydroxyapatite for Biomedical Applications

Published on: February 23, 2017

DNA adsorbed on hydroxyapatite surfaces.

Luis J Del Valle¹, Oscar Bertran, Gustavo Chaves

¹Departament d'Enginyeria Química, E. T. S. d'Enginyeria Industrial de Barcelona, Universitat Politècnica de Catalunya, Diagonal 647, 08028 Barcelona, Spain. jordi.puiggali@upc.edu carlos.aleman@upc.edu.

Journal of Materials Chemistry. B

|April 9, 2020

Summary

This summary is machine-generated.

Hydroxyapatite particle surface properties influence DNA interactions. Surface charge affects DNA adsorption and nuclease protection, while chemical composition impacts buffering capacity and transfection efficacy.

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Area of Science:

Biomaterials science
Surface chemistry
Molecular dynamics simulations

Background:

Hydroxyapatite (HAp) is a key biomaterial.
HAp particle properties vary with synthesis conditions.
Understanding HAp-DNA interactions is crucial for biomaterial applications.

Purpose of the Study:

To investigate how HAp surface charge and composition affect DNA adsorption and protection.
To elucidate the microscopic mechanisms of HAp-DNA interactions using simulations.
To correlate HAp surface characteristics with DNA binding and transfection efficacy.

Main Methods:

Chemical precipitation to synthesize HAp particles with varied properties.
Experimental assessment of DNA adsorption, nuclease protection, and buffering capacity.
Atomistic molecular dynamics simulations of HAp surfaces and adsorbed DNA.

Main Results:

Surface charge influences DNA adsorption and nuclease protection.
Chemical composition affects HAp buffering capacity.
Simulations reveal DNA structural preservation on (001) HAp, but deformation on (010) HAp.
Binding energies correlate with experimental DNA adsorption and protection data.

Conclusions:

HAp surface properties significantly modulate DNA interactions.
Specific HAp crystallographic planes exhibit distinct effects on DNA structure and binding.
Chemical composition is more critical than surface charge for transfection efficacy.