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Real-time Monitoring of Ligand-receptor Interactions with Fluorescence Resonance Energy Transfer

Real-time Monitoring of Ligand-receptor Interactions with Fluorescence Resonance Energy Transfer

Published on: August 20, 2012

A rhodamine B-based lysosomal pH probe.

Shi-Li Shen¹, Xin-Peng Chen, Xiao-Fan Zhang

¹Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, P. R. China. bxzhao@sdu.edu.cn.

Journal of Materials Chemistry. B

|April 9, 2020

Summary

This summary is machine-generated.

A new fluorescent probe, RML, effectively detects acidic conditions in lysosomes. This rhodamine B-based probe shows high selectivity and can visualize lysosomal pH changes in living cells.

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Area of Science:

Biochemistry
Cell Biology
Chemical Biology

Background:

Lysosomal pH is crucial for cellular processes.
Existing probes may lack selectivity or require specific conditions.
Rhodamine B dyes offer pH-dependent fluorescence properties.

Purpose of the Study:

To develop a novel, selective fluorescent probe for monitoring lysosomal pH.
To characterize the probe's response to acidic environments.
To validate the probe's utility in live-cell imaging.

Main Methods:

Synthesis of a rhodamine B-based probe (RML) with a lysosome-targeting moiety.
Evaluation of probe response to pH variations and selectivity against biomolecules.
Live-cell imaging experiments in HeLa cells to visualize lysosomes.

Main Results:

RML demonstrated rapid response to acidic pH at low concentrations.
The probe exhibited excellent membrane permeability and selectivity.
An 80-fold fluorescence increase was observed between pH 7.40 and 4.00 (pKa = 5.16).
RML successfully stained lysosomes in live HeLa cells and detected induced pH changes.

Conclusions:

RML is a valuable tool for studying intracellular acidic organelles, particularly lysosomes.
The probe's characteristics make it suitable for real-time monitoring of lysosomal pH dynamics.
RML facilitates the investigation of cellular processes affected by lysosomal pH.