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Silica@proton-alginate microreactors: a versatile platform for cell encapsulation.

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  • 1Laboratorio de Superficies y Materiales Funcionales INQUIMAE-DQIAQF, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria Pab. II, C1428EHA, Buenos Aires, Argentina. jobbag@qi.fcen.uba.ar.

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Summary
This summary is machine-generated.

Proton-driven alginate gelation offers a novel method for encapsulating live cells, creating a protective hydrogel shell. This technique enables cell survival and preserves their biosynthetic capabilities within an inorganic matrix.

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Area of Science:

  • Biomaterials Science
  • Biotechnology
  • Chemical Engineering

Background:

  • Calcium(II)-alginate encapsulation is a common method for cell immobilization.
  • A novel proton-driven alginate gelation process offers an alternative approach.
  • Investigating its potential as a culture carrier is crucial for biotechnological applications.

Purpose of the Study:

  • To explore proton-driven alginate gelation as a method for cell encapsulation.
  • To assess its efficacy as a culture carrier, both independently and within an inorganic matrix.
  • To evaluate cell survival and functional preservation post-encapsulation.

Main Methods:

  • Proton-driven alginate gelation was employed to form hydrogel shells.
  • Control over gelation front velocity allowed for liquid core formation.
  • A sol-gel process was used to embed capsules within an inorganic host.
  • Cell viability and growth were monitored post-encapsulation.

Main Results:

  • The proton-driven process successfully formed hydrogel shells with liquid cores, protecting cells.
  • Encapsulated cells, including bacteria and microalgae, survived the acidic conditions.
  • Embedded capsules spontaneously redissolved in the inorganic matrix without complexing agents.
  • Cell cultures recovered their initial counts within two weeks, demonstrating viability.
  • Biosynthesis of gold nanoparticles by microalgae confirmed preserved cellular functions.

Conclusions:

  • Proton-driven alginate gelation is a viable alternative for cell encapsulation, preserving cell viability.
  • This method supports cell growth and maintains biosynthetic capabilities.
  • The technique is suitable for creating cell-laden inorganic materials for biotechnological applications.