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Updated: Dec 24, 2025
Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen
Published on: June 3, 2018
A novel biosensor for copper(ii) ions based on turn-on resonance light scattering of ssDNA templated silver
Da-Qian Feng1, Guoliang Liu, Wei Wang
1School of Chemical and Biological Engineering, Yancheng Institute of Technology, Jiangsu 224051, China. liuguoliang188@126.com wangw@ycit.edu.cn.
Abstract:
A new ultrasensitive biosensor for copper(ii) ions was developed based on turn-on resonance light scattering (RLS) of ssDNA templated silver nanoclusters through anti-galvanic reduction (AGR). In our experimental assay, ultra-small size fluorescent C-rich ssDNA templated silver nanoclusters (C-rich ssDNA-Ag NCs) exhibit direct reduction of copper(ii) ions to elemental copper (Cu0) and the resulting Cu0 formed copper nanoparticles on the surface of C-rich ssDNA-Ag NCs spontaneously. The process produced Ag/Cu alloy nanoparticles with larger diameters, turning-on RLS signal of C-rich ssDNA-Ag NCs. As the concentration of Cu2+ ions increased, the RLS signal of C-rich ssDNA-Ag NCs gradually enhanced. The present method achieves the detection of Cu2+ ions in a linear range of 5 × 10-9 M to 7.5 × 10-7 M with a detection limit of 2 nM. Moreover, RLS spectrum, TEM image, fluorescence spectrum, X-ray photoelectron spectroscopy (XPS), and MALDI-TOF mass spectrometry were carried out for investigating the mechanism of the biosensor. The present strategy of constructing a biosensor based on AGR of metal nanoclusters paves a new way to design novel RLS probes.
