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Updated: Dec 24, 2025
Sensing of Barrier Tissue Disruption with an Organic Electrochemical Transistor
Published on: February 10, 2014
Phase angle spectroscopy on transparent conducting polymer electrodes for real-time measurement of epithelial barrier
S Löffler1, A Richter-Dahlfors
1Swedish Medical Nanoscience Center, Department of Neuroscience, Karolinska Institutet, 17177, Stockholm, Sweden. Agneta.Richter.Dahlfors@ki.se.
Abstract:
To facilitate automated real-time monitoring of the formation or disruption of an epithelial monolayer, phase angle spectroscopy was performed in custom-designed cell culture dishes including a transparent conductive polymer-based 2-electrode device. A peak in φ amplitude at 103-105 Hz specifically correlated to increased capacitance by proliferating cells. Dynamic monitoring of cell density, and barrier integrity during monolayer formation and disruption were investigated in combination with live-cell microscopy.
