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Updated: Dec 24, 2025
IridiumIII Luminescent Probe for Detection of the Malarial Protein Biomarker Histidine Rich Protein-II
Published on: July 7, 2015
An iridium oxide nanoparticle and polythionine thin film based platform for sensitive Leishmania DNA detection
Carmen C Mayorga-Martinez1, Alejandro Chamorro-García, Lorena Serrano
1Nanobioelectronics & Biosensors Group, Institut Catala de Nanociencia i Nanotecnologia (ICN2), Edifici ICN2, Campus UAB, Bellaterra (Barcelona) 08193, Spain. arben.merkoci@icn.cat.
Abstract:
An impedimetric label-free genosensor for high sensitive DNA detection is developed. This system is based on a screen-printed carbon electrode modified with the thionine layer and iridium oxide nanoparticles (IrO2 NP). An aminated oligonucleotide probe is immobilized on the IrO2 NP/polythionine modified electrode and ethanolamine was used as a blocking agent. Different diluted PCR amplified DNA samples have been detected. The selectivity and reproducibility of this system are studied and the system was highly reproducible with RSD ≈ 15% and sensitive enough while using 2% of ethanolamine during the blocking step employed for genosensor preparation.
