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Updated: Dec 24, 2025

Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking
Published on: June 8, 2020
An amphiphilic squarylium indocyanine dye for long-term tracking of lysosomes
Chendong Ji1, Yang Zheng, Jie Li
1State Key Laboratory of Chemical Resource Engineering, Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, 100029 Beijing, China. yinmz@mail.buct.edu.cn.
Abstract:
A novel amphiphilic squarylium indocyanine (LysoCy) is reported for remarkable lysosome tracking in live cells. LysoCy performs as a promising lysosome tracker with low cytotoxicity, a strong binding affinity and clear subcellular labelling. The long-term dynamics of lysosomes can be revealed by LysoCy for up to 2 days of culture, while the working duration of commercial dye is no longer than 1 hour. Our work provides a good alternative tool for long-term live cell imaging.
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