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Updated: Dec 24, 2025

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms
Published on: April 17, 2017
High-efficiency immunoassay platforms with controllable surface roughness and oriented antibody immobilization
Lingjie Song1, Jie Zhao, Shifang Luan
1State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China. sfluan@ciac.ac.cn.
Abstract:
High-efficiency immunoassay platforms with controlled surface roughness (single- and dual-scale structured surface) were prepared by combining a facile layer-by-layer particle deposition approach with oriented immobilization of antibodies through boronic acid moieties. The as-prepared surfaces showed significantly enhanced antibody loading capacity and antigen recognition, as proved by fluorescence images.
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