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Platelet-Derived Extracellular Vesicle Functionalization of Ti Implants
Published on: August 5, 2021
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Biofunctionalization of titanium implant with chitosan/siRNA complex through loading-controllable and time-saving
Wen Song1, Lingzhou Zhao, Kaixiu Fang
1State key Laboratory of Military Stomatology, Department of Prosthetic Dentistry, School of Stomatology, The Fourth Military Medical University, No. 145 West Changle Road, Xi'an 710032, China. wqtzym@fmmu.edu.cn.
Journal of Materials Chemistry. B
|April 9, 2020
Summary
This study developed a novel chitosan/siRNA coating on titanium implants using cathodic electrodeposition. This method enhances siRNA delivery for improved bone implant performance and osteogenic differentiation.
Area of Science:
- Biomaterials Science
- Nanotechnology
- Regenerative Medicine
Background:
- Titanium (Ti) implants are widely used but require performance enhancements.
- Small interfering RNA (siRNA) biofunctionalization offers a novel strategy for implant improvement.
Purpose of the Study:
- To develop and evaluate a chitosan (CS)/siRNA complex biofunctionalization method on titania nanotube arrays (TNT) on Ti via cathodic electrodeposition (CED).
- To assess the efficacy of this method for targeted gene silencing and enhancing osteogenic differentiation for bone implant applications.
Main Methods:
- Fabrication of CS/siRNA complex on TNT/Ti using CED.
- Quantification of siRNA deposition and assessment of siRNA release kinetics.
- In vitro evaluation of siRNA delivery efficiency, gene silencing (siGFP), cytocompatibility, and osteogenic differentiation (siCkip-1) in rat mesenchymal stem cells (rMSCs).
Main Results:
- siRNA deposition amount was linearly correlated with current density; release was pH-sensitive.
- CED demonstrated high siRNA delivery efficiency and sustained gene down-regulation, outperforming conventional nanoparticle systems.
- CS/siRNA biofunctionalization showed good cytocompatibility, promoting cell adhesion and viability.
- CS/siCkip-1 enhanced osteogenic differentiation of rMSCs, evidenced by increased osteogenesis gene expression, collagen secretion, and calcium deposition.
Conclusions:
- CED provides a convenient and effective method for biofunctionalizing biomedical metal implants with CS/siRNA for surface activation via local siRNA delivery.
- This approach shows significant promise for enhancing the clinical performance of bone implants by promoting osteogenic differentiation.
