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Updated: Dec 24, 2025

In Vivo Two-Color 2-Photon Imaging of Genetically-Tagged Reporter Cells in the Skin
Published on: July 11, 2019
A membrane-permeable dye for living cells with large two-photon excited fluorescence action cross-sections for
Ruiqing Feng1, Yuming Sun, Minggang Tian
1Center of Bio & Micro/Nano Functional Materials, State Key Laboratory of Crystal Materials, Shandong University, Jinan 250100, P. R. China. yuxq@sdu.edu.cn.
Abstract:
The development of two-photon fluorophores remains an important issue. Dyes that possess both large two-photon excited fluorescence action cross-sections and cell membrane permeability are especially in demand to maximize the underlying virtue of two-photon microscopy for bioimaging. Herein, a novel two-photon excited fluorescence dye has been synthesized. This V-shaped dye exhibited large two-photon excited fluorescence action cross-sections and high plasma membrane permeability. Cell imaging experiments demonstrated that the dye could stain living cells with bright two-photon excited fluorescence. All the results have indicated the potential of the dye as a basic platform for the development of two-photon excited fluorescence probes.
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