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Related Concept Videos

Phosphorylation01:02

Phosphorylation

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The addition or removal of phosphate groups from proteins is the most common chemical modification that regulates cellular processes. These modifications can affect the structure, activity, stability, and localization of proteins within cells as well as their interactions with other proteins.
During phosphorylation, protein kinases transfer the terminal phosphate group of ATP to specific amino acid side chains of substrate proteins. Serine, threonine, and tyrosine are the most commonly...
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Phosphodiester Linkages01:01

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Overview
Phosphodiester bond forms when a phosphoric acid molecule (H3PO4) links with two hydroxyl groups (–OH) of two other molecules, forming two ester bonds. Two water molecules are released in this process. The phosphodiester bond is commonly found in nucleic acids (DNA and RNA) and plays a critical role in their structure and function.
Phosphodiester Bonds Link Nucleotides Together
DNA and RNA are polynucleotides or long chains of nucleotides that are linked together. A nucleotide is...
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Phosphorylated poly(sebacoyl diglyceride) - a phosphate functionalized biodegradable polymer for bone tissue

Peng Huang1, Xiaoping Bi, Jin Gao

  • 1State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, 2999 North Renmin Road, Shanghai 201620, P. R. China. zyou@dhu.edu.cn.

Journal of Materials Chemistry. B
|April 9, 2020
PubMed
Summary
This summary is machine-generated.

A new biodegradable phosphorylated polymer, poly(sebacoyl diglyceride) phosphate (PSeD-P), effectively promotes bone regeneration by enhancing osteoblast activity and inducing mineralization, offering a promising biomaterial for tissue engineering.

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Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Polymer Chemistry

Background:

  • Phosphorylated polymers mimic bone extracellular matrix (ECM) for bone regeneration.
  • Existing synthetic phosphorylated polymers often lack full biodegradability, limiting their tissue engineering applications.

Purpose of the Study:

  • To design and synthesize a novel, biodegradable phosphorylated polymer, poly(sebacoyl diglyceride) phosphate (PSeD-P), for bone regeneration.
  • To evaluate the osteogenic potential of PSeD-P by assessing its effects on osteoblasts and mesenchymal stem cells (MSCs).

Main Methods:

  • Synthesis of PSeD-P based on a biodegradable PSeD backbone, incorporating β-glycerol phosphate (β-GP).
  • Fabrication of 3D porous scaffolds with interconnected macropores and micropores.
  • In vitro assessment of PSeD-P's effects on osteoblast adhesion, proliferation, maturation, and MSC osteogenic differentiation, including biomarker expression and mineralization.

Main Results:

  • PSeD-P demonstrated good biodegradability and was fabricated into porous 3D scaffolds.
  • PSeD-P significantly enhanced osteoblast adhesion, proliferation, and maturation compared to PLGA.
  • PSeD-P induced higher osteogenic biomarker expression and ALP activity in MSCs than its precursor, PSeD, and promoted ECM mineralization without external β-GP.

Conclusions:

  • PSeD-P is the first polymer to integrate the osteoinductive moiety β-GP, offering a biodegradable and osteoconductive material for bone regeneration.
  • PSeD-P shows significant potential for bone regeneration applications by creating an instructive environment for bone formation.
  • The versatile synthesis of PSeD-P allows for tailoring to create a family of phosphorylated polymers for diverse biomedical uses.