Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Design and Manufacturing Principles of Smart Fibers.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

SORBS2 regulates diastolic function through cytoskeletal networks and calcium handling.

Communications biology·2026
Same author

Small Diameter Vascular Grafts Made in Minutes.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Stroke volume analog on a chip - <i>in vitro</i> hydrodynamic model of cardiac pumping efficiency.

Lab on a chip·2026
Same author

BIOPOINT: A particle-based model for probing nuclear mechanics and cell-ECM interactions via experimentally derived parameters.

PLoS computational biology·2026
Same author

A 250-Year Perspective on U.S. Army-Driven Materials Science and Engineering Innovation and Leadership Development.

ACS applied materials & interfaces·2026
Same journal

Multifunctional polysulfone/strontium silicate composites with tunable properties through silane functionalization.

Journal of materials chemistry. B·2026
Same journal

Melanocortin 1 receptor-targeted peptide-functionalized liposomes for enhanced melanocyte-preferential drug delivery and anti-melanogenic efficacy.

Journal of materials chemistry. B·2026
Same journal

Recent progress in side-chain amino acid-based polymers: synthesis, self-assembly, and emerging biomedical applications.

Journal of materials chemistry. B·2026
Same journal

Bioinspired electrospun nanofibrous dressings loaded with Mentha-derived exosome-like vesicles for antibacterial and immunomodulatory burn healing.

Journal of materials chemistry. B·2026
Same journal

On demand functionality of an NIR-enhanced nanozyme catalyst for infected wound healing.

Journal of materials chemistry. B·2026
Same journal

Positively charged, phenolic hydroxyl and anthraquinone structured polystyrene microspheres targeting dual elimination of bacterial pathogens and pathogen-associated molecular patterns for sepsis therapy.

Journal of materials chemistry. B·2026
See all related articles

Related Experiment Video

Updated: Dec 24, 2025

Human iPSC-Derived Cardiomyocyte Networks on Multiwell Micro-electrode Arrays for Recurrent Action Potential Recordings
08:53

Human iPSC-Derived Cardiomyocyte Networks on Multiwell Micro-electrode Arrays for Recurrent Action Potential Recordings

Published on: July 15, 2019

12.0K

Laminar ventricular myocardium on a microelectrode array-based chip.

Ville J Kujala1, Francesco Silvio Pasqualini, Josue A Goss

  • 1Disease Biophysics Group, Harvard Stem Cell Institute, Wyss Institute for Biologically Inspired Engineering, John A. Paulson School of Engineering and Applied Sciences, Harvard University, K.K.P. 29 Oxford Street, Pierce Hall Cambridge, MA 02130, USA. kkparker@seas.harvard.edu.

Journal of Materials Chemistry. B
|April 9, 2020
PubMed
Summary
This summary is machine-generated.

This study developed a novel method using topographical cues to create human cardiac tissues from stem cells. This platform enables accurate drug screening and forms the basis for future heart-on-a-chip systems.

More Related Videos

Laser-Induced Action Potential-Like Measurements of Cardiomyocytes on Microelectrode Arrays for Increased Predictivity of Safety Pharmacology
10:41

Laser-Induced Action Potential-Like Measurements of Cardiomyocytes on Microelectrode Arrays for Increased Predictivity of Safety Pharmacology

Published on: September 13, 2022

2.4K
Microelectrode Array Recording of Sinoatrial Node Firing Rate to Identify Intrinsic Cardiac Pacemaking Defects in Mice
09:20

Microelectrode Array Recording of Sinoatrial Node Firing Rate to Identify Intrinsic Cardiac Pacemaking Defects in Mice

Published on: July 5, 2021

3.4K

Related Experiment Videos

Last Updated: Dec 24, 2025

Human iPSC-Derived Cardiomyocyte Networks on Multiwell Micro-electrode Arrays for Recurrent Action Potential Recordings
08:53

Human iPSC-Derived Cardiomyocyte Networks on Multiwell Micro-electrode Arrays for Recurrent Action Potential Recordings

Published on: July 15, 2019

12.0K
Laser-Induced Action Potential-Like Measurements of Cardiomyocytes on Microelectrode Arrays for Increased Predictivity of Safety Pharmacology
10:41

Laser-Induced Action Potential-Like Measurements of Cardiomyocytes on Microelectrode Arrays for Increased Predictivity of Safety Pharmacology

Published on: September 13, 2022

2.4K
Microelectrode Array Recording of Sinoatrial Node Firing Rate to Identify Intrinsic Cardiac Pacemaking Defects in Mice
09:20

Microelectrode Array Recording of Sinoatrial Node Firing Rate to Identify Intrinsic Cardiac Pacemaking Defects in Mice

Published on: July 5, 2021

3.4K

Area of Science:

  • Biomedical Engineering
  • Stem Cell Biology
  • Cardiovascular Research

Background:

  • Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) are valuable for drug screening but lack native myocardial structure.
  • Current hiPSC-CM models on multi-electrode arrays (MEAs) do not fully replicate the mechanical and structural properties of the human heart.
  • There is a need for advanced in vitro models that better mimic native cardiac tissue for drug development.

Purpose of the Study:

  • To develop a method for fabricating laminar cardiac tissues from hiPSC-CMs that recapitulate native heart architecture.
  • To validate a platform for electrophysiological assessment of drug effects using these engineered cardiac tissues.
  • To integrate this platform with microfluidics for a heart-on-a-chip system.

Main Methods:

  • Utilized topographical cues on soft micromolded gelatin to guide hiPSC-CM self-assembly into laminar tissues.
  • Recorded tissue-level electrophysiological responses using a commercially available multi-electrode array (MEA) setup.
  • Validated the platform by assessing responses to isoproterenol, terfenadine, and fexofenadine, and integrated microfluidic components.

Main Results:

  • Successfully coaxed hiPSC-CMs to form laminar cardiac tissues mimicking native heart architecture.
  • Demonstrated the ability to record tissue-level electrophysiological responses and drug effects using MEAs.
  • Showcased the platform's capability to predict drug responses, including cardiotoxicity, and integrated microfluidics for a heart-on-a-chip system.

Conclusions:

  • Topographical cues on soft substrates enable the formation of biomimetic human cardiac tissues from hiPSC-CMs.
  • This engineered tissue platform provides a robust system for electrophysiological drug screening and safety assessment.
  • The developed heart-on-a-chip system holds promise for advanced drug discovery and personalized medicine.