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Related Concept Videos

siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

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Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
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Related Experiment Video

Updated: Dec 24, 2025

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery
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Nanoparticle delivery systems for siRNA-based therapeutics.

Jinming Li1, Shanshan Xue, Zong-Wan Mao

  • 1MOE Key Laboratory of Bio-inorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China. cesmzw@mail.sysu.edu.cn.

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RNA interference (RNAi) uses small interfering RNA (siRNA) for gene silencing. Nanoparticle delivery systems are advancing efficient in vivo siRNA therapeutics for diseases like cancer.

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Nanomedicine

Background:

  • RNA interference (RNAi) is a natural gene regulation process.
  • Small interfering RNA (siRNA) offers therapeutic potential for genetic diseases.
  • Effective in vivo delivery of siRNA remains a significant challenge.

Purpose of the Study:

  • To review recent advancements in nanoparticle delivery systems for siRNA therapeutics.
  • To highlight the role of nanotechnology in overcoming siRNA delivery barriers.

Main Methods:

  • Review of current literature on nanoparticle-mediated siRNA delivery.
  • Summary of progress in nanotechnology for targeted gene silencing.

Main Results:

  • Nanoparticle systems show promise for efficient intracellular siRNA delivery.
  • Recent advances facilitate targeted and effective in vivo siRNA therapeutics.

Conclusions:

  • Nanotechnology is crucial for the advancement of siRNA-based therapies.
  • Nanoparticle delivery systems are key to realizing the therapeutic potential of siRNA.