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Updated: Dec 24, 2025
Detection of Total Reactive Oxygen Species in Adherent Cells by 2',7'-Dichlorodihydrofluorescein Diacetate Staining
Published on: June 23, 2020
An ultrafast responsive BODIPY-based fluorescent probe for the detection of endogenous hypochlorite in live cells
Liang Qiao1, Hailiang Nie, Yulong Wu
1Key Laboratory of Cluster Science of Ministry of Education, Beijing Key Laboratory of Photoelectronic/Electrophotonic Conversion Materials, Analytical and Testing Center, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 100081, P. R. China. zhangxl@bit.edu.cn hellojane@bit.edu.cn.
Abstract:
Hypochlorite plays a significant role in various physiological and pathological processes; however, its role is still less clear than the role of other reactive oxygen species. Herein, we report an ultrafast responsive (<0.2 s) and highly selective probe B-Ts for hypochlorite with high sensitivity and a detection limit as low as 7.5 nM. The probe was compatible in a wide pH range of 4-13. More importantly, experiments in live cells showed that the probe could penetrate the cell membrane easily and was capable of imaging endogenous and exogenous hypochlorite specifically with a fast response.
