Related Experiment Video
Updated: Aug 10, 2026

Optimized Protocol for Efficient Transfection of Dendritic Cells without Cell Maturation
Published on: July 8, 2011
Enhanced siRNA delivery of a cyclododecylated dendrimer compared to its linear derivative
Wanwan Shen1, Hongmei Liu, Ye Ling-Hu
1Shanghai Key Laboratory of Regulatory Biology, School of Life Sciences, East China Normal University, Shanghai, 200241, P. R. China. 52111300032@ecnu.cn yycheng@mail.ustc.edu.cn.
Abstract:
Lipid modification has been widely applied to improve the gene delivery efficacy of cationic polymers. However, this strategy is often associated with relatively low efficacy and high toxicity due to the inherent features of lipids. Here, we describe a novel and facile strategy to develop polymeric gene vectors via cyclododecylation of cationic dendrimers. The synthesized cyclododecylated dendrimer is much more efficient and biocompatible than the dodecylated analogues.

