Related Experiment Video
Updated: Dec 24, 2025
Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry
Published on: March 1, 2013
Alkylated branched poly(β-amino esters) demonstrate strong DNA encapsulation, high nanoparticle stability and robust
Shuai Liu1, Zhibing Sun, Dezhong Zhou
1Key Laboratory of Functional Polymer Materials, Ministry of Education, Institute of Polymer Chemistry, College of Chemistry, Nankai University, 300071, Tianjin, China. tyguo@nankai.edu.cn.
Abstract:
A branched poly(β-amino ester) with numerous alkyl chains (BPA) is designed and synthesized as a safe and efficient non-viral vector. The branching and hydrophobicity synergistically endow BPA with tight DNA condensation, high polyplex stability in serum, high cellular uptake and ultimately robust gene transfection efficiency, largely superior to its linear counterpart (LPA). Our results demonstrate that branching matters for gene delivery by hydrophobic gene vectors.
