Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Exon Recombination02:32

Exon Recombination

4.0K
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
Exon shuffling follows “splice frame rules.” Each exon...
4.0K
RNA Splicing01:32

RNA Splicing

60.1K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
60.1K
Yeast Signaling01:28

Yeast Signaling

17.0K
Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
17.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A 60-year journey with a fungal transporter: from classical genetics to functional, structural, and evolutionary insights.

Microbiology and molecular biology reviews : MMBR·2026
Same author

Extracellular Phosphate Availability Impacts <i>Aspergillus terreus</i> Itaconic Acid Fermentation via Biomass-Specific Product Yield.

Journal of fungi (Basel, Switzerland)·2026
Same author

Relative contribution of three transporters to D-xylose uptake in <i>Aspergillus niger</i>.

AIMS microbiology·2026
Same author

Propagation of [D1,2]-type spliceosomal twin introns (stwintrons) in <i>Hypoxylaceae</i> and <i>Xylariaceae</i> fungi.

Microbiology spectrum·2025
Same author

CRISPR-Cas9 genome editing in <i>Corallochytrium limacisporum</i>,a key species for understanding animal origins.

Open biology·2025
Same author

Screens for mutants defective in UapA trafficking highlight the importance of ER-exit as a primary control point in transporter biogenesis.

Fungal genetics and biology : FG & B·2024

Related Experiment Video

Updated: Dec 24, 2025

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast
07:31

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

Published on: June 30, 2022

2.8K

Complex intron generation in the yeast genus Lipomyces.

Norbert Ág1, Napsugár Kavalecz1,2, Fruzsina Pénzes1,2

  • 1Dept. of Biochemical Engineering, Faculty of Science and Technology, University of Debrecen, Debrecen, 4032, Hungary.

Scientific Reports
|April 9, 2020
PubMed
Summary

Complex introns called [D] stwintrons, where one U2 intron is nested within another, were studied in yeast. This research suggests a stepwise mechanism for their evolution involving new splice sites and donor element duplication.

More Related Videos

Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production
10:10

Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production

Published on: September 20, 2016

14.8K
In Vivo Monitoring of Transcriptional Activity During Metabolic Transition Using a Bioluminescent Reporter in Yeast
06:53

In Vivo Monitoring of Transcriptional Activity During Metabolic Transition Using a Bioluminescent Reporter in Yeast

Published on: February 21, 2025

834

Related Experiment Videos

Last Updated: Dec 24, 2025

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast
07:31

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

Published on: June 30, 2022

2.8K
Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production
10:10

Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production

Published on: September 20, 2016

14.8K
In Vivo Monitoring of Transcriptional Activity During Metabolic Transition Using a Bioluminescent Reporter in Yeast
06:53

In Vivo Monitoring of Transcriptional Activity During Metabolic Transition Using a Bioluminescent Reporter in Yeast

Published on: February 21, 2025

834

Area of Science:

  • Molecular Biology
  • Genetics
  • Evolutionary Biology

Background:

  • Eukaryotic nuclear genes contain U2-type introns that interrupt coding sequences.
  • Complex introns can be excised through consecutive splicing reactions, posing challenges to understanding intron origins.
  • [D] stwintrons represent a class of complex introns with a nested U2 intron structure.

Purpose of the Study:

  • To investigate the structure and evolution of complex U2-type introns in the yeast genus Lipomyces.
  • To elucidate the mechanism of [D] stwintron formation and excision.
  • To explore the evolutionary origins of spliceosomal introns.

Main Methods:

  • Comparative sequence analysis of introns across different Lipomyces species (L. lipofer, L. suomiensis, L. starkeyi).
  • Identification and characterization of nested U2 intron units within complex intervening sequences.
  • Analysis of alternative splicing pathways in L. starkeyi.

Main Results:

  • Three distinct complex intervening sequences with nested U2 intron units were identified in the reticulon-like protein gene of Lipomyces species.
  • In L. starkeyi, a complex intron exhibited abutting donor elements, allowing for alternative excision via a single standard splicing reaction or a two-step [D] stwintron pathway.
  • Evidence suggests [D] stwintrons can arise from the emergence of new splice sites within existing introns.

Conclusions:

  • The study proposes a stepwise mechanism for [D] stwintron evolution, potentially involving duplication of the ancestral intron's donor element.
  • This mechanism provides insights into how complex introns may emerge and evolve.
  • Understanding these complex introns contributes to the broader question of spliceosomal intron origins.