Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

The Mammary Glands

The Mammary Glands

The female breast is a hemispheric projection of variable size positioned anterior to the pectoralis major and serratus anterior muscles. A fascia layer composed of dense, irregular connective tissue connects it to these muscles.
Each breast features a pigmented projection known as the nipple, through which milk emerges via closely spaced openings of ducts, referred to as lactiferous ducts. Surrounding the nipple is a circular pigmented area of skin named the areola, which appears rough due to...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Induction of senescence during postpartum mammary gland involution supports tissue remodeling and promotes postpartum tumorigenesis.

Nature aging·2026

Same author

Modeling Mammary Gland Development and Reproductive Cycles With Organoids.

Biology of the cell·2025

Same author

In Vivo Risk Assessment of Yellow Fever Virus Transmission Through Breastfeeding, and Mechanistic Insights.

The Journal of infectious diseases·2025

Same author

Epigenomic disorder and partial EMT impair luminal progenitor integrity in Brca1-associated breast tumorigenesis.

Molecular cancer·2025

Same author

Author Correction: Systemic GDF11 attenuates depression-like phenotype in aged mice via stimulation of neuronal autophagy.

Nature aging·2023

Same author

Systemic GDF11 attenuates depression-like phenotype in aged mice via stimulation of neuronal autophagy.

Nature aging·2023

Search research articles

Related Experiment Video

Updated: Dec 24, 2025

Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland

Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland

Published on: December 1, 2015

Primary Mammary Organoid Model of Lactation and Involution.

Jakub Sumbal^1,2, Aurelie Chiche¹, Elsa Charifou¹

¹Department of Developmental and Stem Cell Biology, Cellular Plasticity and Disease Modelling, CNRS UMR 3738, Institut Pasteur, Paris, France.

Frontiers in Cell and Developmental Biology

|April 9, 2020

Summary

Researchers developed a novel mouse mammary organoid system to model lactation and involution. This 3D model advances the study of mammary gland biology and pregnancy-associated breast cancer.

Keywords:

3D culture fibroblast growth factor 2 involution lactation mammary gland milk production organoid prolactin

More Related Videos

Generation of Mosaic Mammary Organoids by Differential Trypsinization

Generation of Mosaic Mammary Organoids by Differential Trypsinization

Published on: March 11, 2020

Evaluation of Mammary Gland Development and Function in Mouse Models

Evaluation of Mammary Gland Development and Function in Mouse Models

Published on: July 21, 2011

Related Experiment Videos

Last Updated: Dec 24, 2025

Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland

Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland

Published on: December 1, 2015

Generation of Mosaic Mammary Organoids by Differential Trypsinization

Generation of Mosaic Mammary Organoids by Differential Trypsinization

Published on: March 11, 2020

Evaluation of Mammary Gland Development and Function in Mouse Models

Evaluation of Mammary Gland Development and Function in Mouse Models

Published on: July 21, 2011

Area of Science:

Mammary gland biology and development
3D organoid culture systems
Breast cancer research

Background:

Mammary gland development involves puberty, pregnancy, lactation, and involution, with significant tissue remodeling.
Three-dimensional (3D) mammary organoid cultures are valuable for studying pubertal development and breast cancer.
A gap exists in 3D models for studying lactation and involution stages.

Purpose of the Study:

To establish a robust 3D mouse mammary organoid system.
To model key aspects of lactation and involution in vitro.
To provide a tool for investigating mammary gland physiology and disease.

Main Methods:

Development of a straightforward mouse mammary organoid culture system.
Utilizing the system to recapitulate lactation and involution-like processes.
Application of the model for mechanistic studies.

Main Results:

A novel and effective 3D mouse mammary organoid system was established.
The system successfully models lactation and involution-like mammary gland changes.
This model enables the study of physiological and pathological processes.

Conclusions:

The developed mammary organoid system effectively models lactation and involution.
This 3D culture system is a valuable tool for mammary gland research.
The model has implications for understanding pregnancy-associated breast cancer.