Silver ion bioreduction in nanoparticles using Artemisia annua L. extract: characterization and application as...

The biological synthesis of metal nanoparticles using plant extracts with defined size and morphology is a simple, nontoxic and environmentally friendly method. The present study focused on the synthesis of silver nanoparticles (Ag NPs) by Artemisia annua L. extract as reducing and stabilising agent. The Ag NPs function, as antibacterial agents, is with that they are further used in human therapy. The effects of pH and temperature on the synthesis of NPs were characterized by UV-absorption spectroscopy and shown by surface plasmon resonance (SPR) band at 410 nm. NPs' size and morphology were measured by transmission electron microscopy (TEM) and dynamic light scattering (DLS). TEM images showed that Ag NPs were in a nano-sized range (20-90 nm) and had spherical shape. Our findings demonstrated that lower concentration (100 µg mL^-1) of the biogenic Ag NPs exhibited antibacterial activity against Gram-negative Escherichia coli BW 25113 and Gram-positive Enterococcus hirae ATCC 9790.