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Updated: Dec 24, 2025

Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor
Published on: April 6, 2016
Partially shielded enzymes capable of processing large protein substrates
Manon L Briand1, Remy Gebleux, Federica Richina
1School of Life Science, University of Applied Sciences and Arts Northwestern Switzerland, Hofackerstrasee 30, Muttenz CH-4132, Switzerland. patrick.shahgaldian@fhnw.ch.
Abstract:
We report the first method of enzyme protection enabling the production of partially shielded enzymes capable of processing substrates as large as proteins. We show that partially shielded sortase retains its transpeptidase activity and can perform bioconjugation reactions on antibodies. Moreover, a partially shielded trypsin is shown to outperform its soluble counterpart in terms of proteolytic kinetics. Remarkably, partial enzyme shielding results in a drastic increase in temporal stability of the enzyme.
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