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Quantitative Formation of Monomeric G-Quadruplex DNA from Multimeric Structures of c-Myc Promoter Sequence
Valerie Rauser1, Elmar Weinhold1
1Institute of Organic Chemistry, RWTH Aachen University, Landoltweg 1, 52074, Aachen, Germany.
Chembiochem : a European Journal of Chemical Biology
|April 9, 2020
Summary
Synthetic DNA studies face challenges with G-quadruplex (G4) forming sequences. This research presents a method to convert problematic multimeric G4 structures into desired monomeric forms using alkaline treatment and refolding.
Area of Science:
- Molecular Biology
- Biochemistry
- Genetics
Background:
- G-quadruplex (G4) forming DNA sequences can aggregate into stable multimeric structures.
- These multimeric structures pose challenges in studies utilizing synthetic oligodeoxynucleotides.
Purpose of the Study:
- To develop a method for converting multimeric G4 structures into monomeric G4.
- To address issues caused by G4 sequence aggregation in synthetic DNA studies.
Main Methods:
- Utilized alkaline treatment to disrupt multimeric G-quadruplex structures.
- Employed refolding techniques to obtain the desired monomeric G-quadruplex conformation.
- Focused on the Pu27 sequence from the c-myc promoter.
Main Results:
- Successfully converted multimeric intermolecular G4 structures into monomeric G4.
- Demonstrated quantitative conversion of the Pu27 G4 sequence.
- Established a reliable method for obtaining monomeric G4 structures.
Conclusions:
- Alkaline treatment and refolding provide an effective method for monomerizing G4 structures.
- This technique is crucial for accurate studies involving synthetic G4-forming DNA sequences.
- Facilitates reliable research on G-rich sequences like the Pu27 from the c-myc promoter.
