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Generation of a TLR7 heterozygous knockout line (WAe009-A-18) from human embryonic stem cells using CRISPR/Cas9
Hyang-Hee Seo1, Hyo-Won Han1, Jung-Hyun Kim1
1Division of Intractable Diseases, Center for Biomedical Sciences, Korea National Institute of Health, Cheongju 28159, Republic of Korea.
Stem Cell Research
|April 9, 2020
Abstract:
Toll-like receptor 7 (TLR7) is a member of the toll-like receptor (TLR) family that is essential in the innate immune system. In this study, we established a heterozygous TLR7 knockout H9 cell line using CRISPR/Cas9. TLR7 knockout H9 cells maintained their pluripotency and exhibited the ability to differentiate into the three germ layers without any karyotype abnormalities.
