BSA loaded bead-on-string nanofiber scaffold with core-shell structure applied in tissue engineering

Beaded nanofiber is a promising fibrous structure could act as drug delivery system with sustained drug release for regulating cell behaviors used in tissue engineering. Poly (L-lactic acid-co-ε-caprolactone) (PLCL) beaded nanofiber with core-shell structure (130 ± 30 nm) was fabricated and bovine serum albumin (BSA) was encapsulated into the inner layer. The surface morphology and characteristic were evaluated by scanning electron microscopy (SEM), inverted fluorescence microscopy and water contact angle test. Degradation analyses suggested that PLCL/BSA core-shell @ beaded nanofibers could maintain the fibrous framework during 3 weeks. The biocompatibility was investigated by in vitro cultivation of human mesenchymal stem cells (hMSCs) on the surface of PLCL/BSA core-shell @ beaded nanofibers. The proliferation of hMSCs was tested using alamar blue reagent and the spreading morphology of cells was observed by SEM. Corresponding results suggested that beaded nanofibers with core-shell structure could effectively support the attachment and proliferation of cells. PLCL beaded nanofiber with core-shell structure would work as a promising candidate for drug release system and tissue engineering.