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A new method for the unidirectional deletion of DNA with Bal 31 nuclease using 2'-O-MeRNA-DNA chimeric adaptors
S Mukai1, S Shibahara, H Morisawa
1Central Research Laboratories, Ajinomoto Co., Inc., Kawasaki, Japan.
Nucleic Acids Symposium Series
|January 1, 1988
Abstract:
We have developed a new procedure for unidirectional DNA deletion with Bal 31 nuclease. This method is based on the use of chimeric adaptors containing double strands of 2'-O-methyl RNA to block against an attack of Bal 31 nuclease. This contributes a wide application to learn the relation of structure-function of genes.