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MicroRNA-29 family inhibits rhabdomyosarcoma formation and progression by regulating GEFT function

Yang Wang1, Liang Zhang1,2, Yuweng Pang1

  • 1Department of Pathology and Key Laboratory for Xinjiang Endemic and Ethnic Diseases, The First Affiliated Hospital, Shihezi University School of Medicine Shihezi 832002, China.

American Journal of Translational Research
|April 10, 2020
PubMed

Insights

MicroRNA-29 (mir-29a/b/c) expression is reduced in rhabdomyosarcoma (RMS). Restoring mir-29 inhibits RMS cell growth, invasion, and tumor formation by targeting GEFT, suggesting mir-29 as a potential therapeutic target for RMS.

Area of Science:

  • Oncology
  • Molecular Biology
  • Gene Regulation

Background:

  • The microRNA-29 family (mir-29a, mir-29b, mir-29c) has a dual role in various cancers.
  • The function of the microRNA-29 family in rhabdomyosarcoma (RMS) remains unclear.

Purpose of the Study:

  • To investigate the role and expression of the microRNA-29 family in rhabdomyosarcoma.
  • To determine if microRNA-29 can serve as a therapeutic target for RMS.

Main Methods:

  • Quantitative real-time PCR to detect mir-29a/b/c expression in RMS tissues and cell lines.
  • Cell proliferation, migration, invasion, and apoptosis assays.
  • Dual-luciferase reporter assay and RNA immunoprecipitation to identify direct targets.
  • In vivo subcutaneous xenograft model in nude mice.

Main Results:

  • MicroRNA-29 family expression was significantly downregulated in RMS tissues and cell lines compared to normal muscle cells.
  • Overexpression of mir-29a/b/c inhibited RMS cell proliferation, migration, invasion, and induced apoptosis.
  • GEFT was identified as a direct target of mir-29a/b/c, and mir-29s weakened GEFT's tumor-promoting effects.
  • Mir-29a inhibited RMS tumor growth in vivo and reduced GEFT expression in xenograft tumors.

Conclusions:

  • MicroRNA-29 family members inhibit rhabdomyosarcoma development and progression.
  • Mir-29a/b/c function as tumor suppressors in RMS by targeting GEFT.
  • MicroRNA-29 represents a potential molecular therapeutic target for rhabdomyosarcoma treatment.
Keywords:
GEFTRhabdomyosarcomaapoptosisinvasionmigrationmir-29a/b/cproliferation

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