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Intraluminal vesicles (ILVs) are small vesicles 50-80 nm in diameter formed during the maturation of early endosomes. A specialized endosome containing numerous ILVs is called a multivesicular body (MVB). ILVs contain internalized molecules such as antigens, nucleic acids, proteins, and metabolites. Some of these molecules are released from the MVBs inside exosomes and are transported to other cells. Other MVBs contain molecules that are retained in the ILVs and are later degraded within the...
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Related Experiment Video

Updated: Dec 24, 2025

Isolation and Analysis of Traceable and Functionalized Extracellular Vesicles from the Plasma and Solid Tissues
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Basic knowledge on BCR-ABL1-positive extracellular vesicles.

Ancuta Jurj1, Sergiu Pasca2, Patric Teodorescu2,3

  • 1Research Center for Functional Genomics, Biomedicine & Translational Medicine, Iuliu Hatieganu University of Medicine & Pharmacy, 23 Marinescu Street, 400337, Cluj-Napoca, Romania.

Biomarkers in Medicine
|April 10, 2020
PubMed
Summary
This summary is machine-generated.

Extracellular vesicles (EVs) in chronic myelogenous leukemia (CML) foster a supportive microenvironment and may help predict remission. Further research is needed to confirm their role in predicting CML relapse.

Keywords:
BCR-ABLcell communicationchronic myeloid leukemiaextracellular vesiclesmicroenvironmentmicrovesiclestranslational medicine

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Area of Science:

  • Hematology
  • Oncology
  • Cell Biology

Background:

  • Chronic myelogenous leukemia (CML) is a myeloid malignancy marked by excessive myeloid progenitor proliferation.
  • Extracellular vesicles (EVs) play a role in intercellular communication within the bone marrow microenvironment.

Purpose of the Study:

  • To investigate the role of BCR-ABL1-positive extracellular vesicles (EVs) in chronic myelogenous leukemia (CML).
  • To explore the potential of these EVs in clinical research for monitoring molecular remission and predicting relapse.

Main Methods:

  • Analysis of extracellular vesicles (EVs) derived from CML patients.
  • Characterization of EVs for the presence of BCR-ABL1.
  • Assessment of EV interactions with various cell types in the hematopoietic system.

Main Results:

  • BCR-ABL1-positive EVs were found to communicate with hematopoietic stem cells, mesenchymal stem cells, myeloid-derived suppressor cells, and endothelial cells.
  • This communication creates a microenvironment conducive to CML clone expansion.
  • BCR-ABL1-positive EVs show potential utility in assessing molecular remission grades.

Conclusions:

  • BCR-ABL1-positive EVs contribute to a favorable microenvironment for CML progression.
  • These EVs may serve as biomarkers for monitoring treatment response and potentially predicting relapse in CML.
  • The study suggests that BCR-ABL1-positive EVs could be a model for understanding other malignancies involving fusion genes within EVs.