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Protocol of proceedings with Fusobacterium nucleatum and optimization of ABTS method for detection of reactive oxygen
Anna Kędziora1, Monika Katarzyna Lesiów2, Katarzyna Krupa2
1Department of Microbiology, Institute of Genetics & Microbiology, University of Wrocław, S. Przybyszewskiego 63, Wrocław 50-001, Poland.
Abstract:
Aim: Characterization of the ability of Fusobacterium nucleatum DSM 15643 and DSM 20482 strains in the presence of Cu2+ and H2O2 to reactive oxygen species generation. Method: Spectrophotometric ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) method was used. Results: Determination of: MIC for Cu2+, H2O2 and ABTS; survivability of F. nucleatum under atmospheric oxygen exposure; the level and rate constants of free radicals production by the bacteria. Conclusion:F. nucleatum in the presence of Cu2+ and H2O2 is able to generate free radicals. Reactive oxygen species are produced mainly outside the bacterial cell, which suggests that outer membrane proteins may be involved in oxidative process.
