Global responses to tris(1-chloro-2-propyl)phosphate (TCPP) in rockfish Sebastes schlegeli using integrated proteomic...

As alternatives of brominated flame retardants, organophosphate flame retardants (OPFRs) can be detected in multiple marine environmental media. Tris(1-chloro-2-propyl)phosphate (TCPP) was one of the most frequently and abundantly detected OPFRs in the Bohai Sea. Exposure to TCPP has been shown to induce abnormal behavior in zebrafish as well as neurotoxicity in Caenorhabditis elegans. However, there is a lack of mechanism investigations on the toxic effects of TCPP at molecular levels. In this work, proteomics and metabolomics were integrated to analyze the proteome and metabolome responses in rockfish Sebastes schlegeli treated with TCPP (10 and 100 nM) for 15 days. A total of 143 proteins and 8 metabolites were significantly altered in rockfish following TCPP treatments. The responsive proteins and metabolites were predominantly involved in neurotransmission, neurodevelopment, signal transduction, cellular transport, cholesterol metabolism, bile acid synthesis, and detoxification. Furthermore, a hypothesized network of proteins, metabolites, and pathways in rockfish was summarized based on the combination of proteomic and metabolomic results, showing some key molecular events in response to TCPP. Overall, the present study unraveled the molecular responses at protein and metabolite levels, which provided a better understanding of toxicological effects and mechanisms of TCPP in marine teleost.