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Updated: Dec 24, 2025

Quantifying the Binding Interactions Between CuII and Peptide Residues in the Presence and Absence of Chromophores
Published on: April 5, 2022
A dual-functional probe for sensing pH change and ratiometric detection of Cu2
Jiang-Xu Wang1, Zhi-Yong Xing2, Zhen-Nan Tian2
1College of Life Science, Northeast Agricultural University, Harbin 150030, PR China.
Abstract:
A series of benzothiazole-based compounds were synthesized and characterized. Among them, probe Z showed significant dual-functional performance which was capable of sensing pH change and Cu2+. Probe Z displayed fluorescent turn-on under alkaline conditions due to deprotonation of the hydroxyl group along with the obviously color change from colorless to mint green. Interestingly, it further achieved in ratiometric detection of Cu2+ through absorbance or fluorescence signals in strong alkaline condition. The limit of detection was calculated correspondingly as 0.37 μM and 1.35 μM, respectively. Especially, the combination of the XNOR and INHIBIT logic gates could be used to confirm that one medium was in neutrality or alkalinity condition. Moreover, Z was successfully used in real water samples and test paper for fast identification of Cu2+, respectively.
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