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Updated: Dec 24, 2025

A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time

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Reference values for thrombin dynamics in platelet rich plasma.

Qiuting Yan^1,2, Marisa Ninivaggi^1,2, Bas de Laat^1,2

¹Department of Funtional Coagulation, Synapse Research Institute, Maastricht, The Netherlands.

|April 11, 2020

Summary

This summary is machine-generated.

Thrombin generation (TG) in platelet-rich plasma (PRP) was studied to establish reference ranges. Platelet count primarily impacts the rate of thrombin generation, not the total amount formed.

Keywords:

Platelet rich plasma prothrombin conversion thrombin dynamics thrombin generation thrombin inactivation

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Published on: April 6, 2017

Area of Science:

Hemostasis and Thrombosis Research
Clinical Coagulation Analysis

Background:

Thrombin generation (TG) is a superior indicator of hemostatic function compared to traditional clotting assays.
Assessing platelet contribution to TG is crucial due to bleeding risks associated with low or dysfunctional platelets.
Platelet-poor plasma is commonly used, but platelet-rich plasma (PRP) offers a more comprehensive view.

Purpose of the Study:

To establish reference ranges for thrombin generation and dynamics in platelet-rich plasma (PRP) from healthy individuals.
To investigate the impact of varying platelet counts on thrombin generation parameters.
To analyze the relationship between platelet count, prothrombin conversion, and thrombin inhibition.

Main Methods:

Collected platelet-rich plasma (PRP) from 117 healthy donors.
Measured TG in undiluted PRP and PRP diluted to various platelet concentrations (10-400 x 10^9/L).
Calculated prothrombin conversion and thrombin inactivation using TG parameters and coagulation factor levels (antithrombin, α2-Macroglobulin, fibrinogen).

Main Results:

Established reference ranges for TG and thrombin dynamics in PRP.
Peak thrombin, velocity index, and maximum prothrombin conversion rate increased linearly with platelet count.
Endogenous thrombin potential peaked at 150 x 10^9/L; platelet counts below 50 x 10^9/L minimally affected most TG parameters (except ETP).

Conclusions:

Thrombin generation and dynamics in PRP were successfully assessed, providing essential reference values.
Increasing platelet count primarily influences the rate of prothrombin conversion and overall TG, not the total thrombin generated.
Inhibitory mechanisms by antithrombin and α2-Macroglobulin scale with TG but maintain a consistent ratio.