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Updated: Dec 24, 2025
Protocol for Developing a Femur Osteotomy Model in Wistar Albino Rats
Published on: August 31, 2022
Robust method to create a standardized and reproducible atrophic non-union model in a rat femur
Tadanobu Onishi1, Takamasa Shimizu1, Manabu Akahane2
1Department of Orthopedic Surgery, Nara Medical University, Kashihara, Nara, 634-8522, Japan.
Objective:
No evidence exists about which biological approach is more reliable for creating non-union model. We investigated how to create a reproducible atrophic non-union model in a rat femur.
Methods:
We compared three groups: simple osteotomy (group A), partial periosteum cauterization (group B), and extensive periosteum and bone marrow resection (group C).
Results:
All samples in group C demonstrated atrophic non-union in radiological, histological, and biomechanical analyses, however half of the samples in group B showed fracture healing at week 16.
Conclusion:
Extensive resection of periosteum and bone marrow is important for a reproducible atrophic non-union model in a rat femur.
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