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CK19 Promotes Ovarian Cancer Development by Impacting on Wnt/β-Catenin Pathway.

Qi Lu1, Hong Qu1, Tong Lou1

  • 1Department of Obstetrics and Gynecology, Chao-yang Hospital, Capital Medical University, Beijing, People's Republic of China.

Oncotargets and Therapy
|April 11, 2020
PubMed
Summary

Cytokeratin 19 (CK19) promotes epithelial ovarian cancer (EOC) progression by enhancing cell invasion, proliferation, and migration. CK19 activates the Wnt/β-catenin pathway, offering a potential therapeutic target for EOC.

Keywords:
CK19SKOV3Wnt/β-cateninovarian cancer

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Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • Epithelial ovarian cancer (EOC) is the most lethal gynecologic malignancy.
  • Cytokeratin 19 (CK19) is a type I cytokeratin with an unexplored role in EOC.

Purpose of the Study:

  • To investigate the functional role of CK19 in EOC.
  • To elucidate the underlying molecular mechanisms of CK19 in EOC progression.

Main Methods:

  • Western blotting and RT-PCR to assess CK19 expression in EOC tissues.
  • Transwell and CCK-8 assays to evaluate cell invasion, migration, and proliferation.
  • Analysis of Wnt/β-catenin pathway genes (β-catenin, TCF7, LEF1, c-MYC, cyclin D1) in transfected ovarian cancer cells.

Main Results:

  • CK19 expression is upregulated in EOC tissues.
  • Overexpression of CK19 promotes ovarian cancer cell invasion, proliferation, and migration.
  • CK19 activates the Wnt/β-catenin signaling pathway, upregulating key target genes.

Conclusions:

  • This study is the first to demonstrate the role of CK19 in EOC.
  • CK19 acts as a promoter of EOC progression via the Wnt/β-catenin pathway.
  • CK19 represents a potential therapeutic target for EOC diagnosis and treatment.