Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The chicken retrovirus-like gene <i>ENS-1/ERNI</i> and <i>Soprano</i> LTR are involved in primordial germ cell development.

iScience·2026

Same author

Switchable photoluminescence of europium(III) complexes with chromonylhydrazones.

Chemical communications (Cambridge, England)·2026

Same author

Lifetime sports and exercise experience are associated with cognitive, social, and resting-state brain activity in adults.

Brain imaging and behavior·2026

Same author

Three-dimensional analysis of maxillary asymmetry in patients with mandibular prognathism.

Maxillofacial plastic and reconstructive surgery·2026

Same author

[Pulmonary Arterial Hypertension Impairs Duodenal Barrier Integrity in Rats].

Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·2026

Same author

ATAC-seq of low-input and cryopreserved primordial germ cells reveals functional enhancers.

Development (Cambridge, England)·2026

Same journal

From contraindication to consideration: the role of esophagectomy in stage IV esophageal adenocarcinoma.

Journal of thoracic disease·2026

Same journal

Clinical features and prognostic factor of small (≤2 cm) lung cancer associated with cystic airspaces.

Journal of thoracic disease·2026

Same journal

Sex-specific trends in incidence and mortality of interstitial lung disease and pulmonary sarcoidosis in China compared with global estimates, 1990-2023: an analysis of GBD 2023.

Journal of thoracic disease·2026

Same journal

A single-arm, single-center phase II clinical study of concurrent brain radiotherapy combined with tislelizumab and chemotherapy in patients with small-cell lung cancer and brain metastases.

Journal of thoracic disease·2026

Same journal

Minimally invasive off-pump coronary artery bypass grafting is associated with reduced postoperative intra-aortic balloon pump use.

Journal of thoracic disease·2026

Same journal

Pulmonary artery involvement contributes to the recurrence of hemoptysis after bronchial artery embolization in patients with chronic pulmonary aspergillosis.

Journal of thoracic disease·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Dec 24, 2025

Inactivation of Pathogens via Visible-Light Photolysis of Riboflavin-5′-Phosphate

Inactivation of Pathogens via Visible-Light Photolysis of Riboflavin-5′-Phosphate

Published on: April 6, 2022

Vitamin B2 lung-marking method using black light irradiation.

Yusuke Tanaka¹, Isao Matsumoto¹, Munehisa Takata¹

¹Department of Thoracic, Cardiovascular and General Surgery, Kanazawa University, Kanazawa, Japan.

Journal of Thoracic Disease

|April 11, 2020

Summary

This summary is machine-generated.

Vitamin B2 injection safely and accurately marked lung lesions in a pig model, offering a potentially safer alternative to the hook wire method for lung tumor resection. Further clinical trials are planned.

Keywords:

Lung-marking black light vitamin B2

More Related Videos

A Functional Whole Blood Assay to Measure Viability of Mycobacteria, using Reporter-Gene Tagged BCG or M.Tb BCG lux/M.Tb lux

A Functional Whole Blood Assay to Measure Viability of Mycobacteria, using Reporter-Gene Tagged BCG or M.Tb BCG lux/M.Tb lux

Published on: September 14, 2011

Assessment of the Cytotoxic and Immunomodulatory Effects of Substances in Human Precision-cut Lung Slices

Assessment of the Cytotoxic and Immunomodulatory Effects of Substances in Human Precision-cut Lung Slices

Published on: May 9, 2018

Related Experiment Videos

Last Updated: Dec 24, 2025

Inactivation of Pathogens via Visible-Light Photolysis of Riboflavin-5′-Phosphate

Inactivation of Pathogens via Visible-Light Photolysis of Riboflavin-5′-Phosphate

Published on: April 6, 2022

A Functional Whole Blood Assay to Measure Viability of Mycobacteria, using Reporter-Gene Tagged BCG or M.Tb BCG lux/M.Tb lux

A Functional Whole Blood Assay to Measure Viability of Mycobacteria, using Reporter-Gene Tagged BCG or M.Tb BCG lux/M.Tb lux

Published on: September 14, 2011

Assessment of the Cytotoxic and Immunomodulatory Effects of Substances in Human Precision-cut Lung Slices

Assessment of the Cytotoxic and Immunomodulatory Effects of Substances in Human Precision-cut Lung Slices

Published on: May 9, 2018

Area of Science:

Thoracic Surgery
Surgical Navigation
Medical Imaging

Background:

Current lung tumor resection relies on methods like hook wire localization.
Hook wire localization, while simple, carries a risk of lethal air embolism.
Vitamin B2 is a safe, fluorescent agent explored for thoracic surgery applications.

Purpose of the Study:

To evaluate vitamin B2 as a replacement for hook wire localization in marking peripheral lung lesions.
To assess the safety and efficacy of vitamin B2 lung-marking in a preclinical pig model.

Main Methods:

A pig model underwent simultaneous hook wire localization and vitamin B2 injection.
Vitamin B2 solution was injected into the lung parenchyma at the hook wire puncture site.
Fluorescence intensity and marked area length were measured over time under black light.

Main Results:

Vitamin B2 successfully marked lung lesions in all pigs, visible under black light.
Marked area length and fluorescence intensity remained stable over 120 minutes.
The vitamin B2-marked area exhibited significantly higher fluorescence compared to normal lung tissue.

Conclusions:

Vitamin B2 lung-marking is a safe and accurate method in a pig model.
The technique provides excellent visibility and may serve as a viable alternative to hook wire localization.
Clinical trials are planned to validate this promising method for human application.