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Using a GFP-tagged TMEM184A Construct for Confirmation of Heparin Receptor Identity
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Heparanase: Cloning, Function and Regulation.

Shaun M Gaskin1, Tatiana P Soares Da Costa1, Mark D Hulett2

  • 1Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Bundoora, VIC, Australia.

Advances in Experimental Medicine and Biology
|April 11, 2020
PubMed
Summary
This summary is machine-generated.

The human heparanase gene was cloned 20 years ago. This enzyme

Keywords:
Enzyme activityGene cloningGene regulationHeparan sulfateHeparanase

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Extracellular Matrix Biology

Background:

  • Heparanase cleaves heparan sulfate, a key extracellular matrix component.
  • Understanding heparanase is crucial for both normal physiology and disease.
  • The 20th anniversary of the human heparanase gene cloning highlights its significance.

Purpose of the Study:

  • To provide a historical overview of the human heparanase gene cloning.
  • To describe the intracellular and extracellular functions of heparanase.
  • To explore regulatory mechanisms of heparanase expression and activity.

Main Methods:

  • Historical literature review.
  • Functional analysis of heparanase.
  • Examination of gene, transcript, and protein regulation.

Main Results:

  • The cloning of the human heparanase gene.
  • Detailed description of heparanase's dual localization and function.
  • Identification of multiple regulatory levels for heparanase.

Conclusions:

  • Heparanase is a unique enzyme with critical roles in health and disease.
  • Regulation occurs at the gene, transcript, and protein levels.
  • Continued research into heparanase mechanisms is essential.