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MicroRNA-1271-5p inhibits cell proliferation and enhances radiosensitivity by targeting CDK1 in hepatocellular

Hong-Mei Liu1,2, Hua-Yan Tan2, Yue Lin1

  • 1Research Department, Affiliated Cancer Hospital of Guangxi Medical University and Cancer Institute of Guangxi Zhuang Autonomous Region, Nanning, Guangxi 530021, P.R. China.

Journal of Biochemistry
|April 11, 2020
PubMed

Insights

MicroRNA-1271-5p (miR-1271-5p) inhibits hepatocellular carcinoma (HCC) cell proliferation and improves radiosensitivity by targeting cyclin-dependent kinase 1 (CDK1). This finding offers a potential therapeutic strategy for HCC treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Hepatocellular carcinoma (HCC) remains a significant global health challenge.
  • Understanding the molecular mechanisms underlying HCC progression and treatment resistance is crucial.
  • MicroRNAs (miRNAs) have emerged as key regulators in various cancers, including HCC.

Purpose of the Study:

  • To investigate the role of miR-1271-5p in hepatocellular carcinoma (HCC).
  • To determine if miR-1271-5p inhibits HCC cell proliferation and enhances radiosensitivity.
  • To identify the direct molecular target of miR-1271-5p in HCC.

Main Methods:

  • Bioinformatics analysis to predict potential miRNA targets.
  • Dual-Luciferase Reporter Assay to validate direct miRNA-target interaction.
  • In vitro cell culture experiments (SMMC-7721 and HuH-7 cells) to assess proliferation and radiosensitivity.
  • In vivo studies using transplanted tumors in nude mice.

Main Results:

  • miR-1271-5p expression was significantly lower in HCC cell lines compared to normal liver cells.
  • Cyclin-dependent kinase 1 (CDK1) was identified as a direct target of miR-1271-5p.
  • Overexpression of miR-1271-5p reduced HCC cell proliferation, enhanced radiosensitivity, altered cell cycle, and inhibited tumor growth in vivo.
  • Restoring CDK1 expression attenuated the radiosensitizing effect of miR-1271-5p.

Conclusions:

  • miR-1271-5p functions as a tumor suppressor in hepatocellular carcinoma.
  • The inhibitory effect of miR-1271-5p on proliferation and its radiosensitizing properties are mediated through the targeting of CDK1.
  • miR-1271-5p represents a potential therapeutic target for improving HCC treatment outcomes.
Keywords:
CDK1hepatocellular carcinomamiR-1271-5pproliferationradiosensitivity

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