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Site-Directed Immobilization of Bone Morphogenetic Protein 2 to Solid Surfaces by Click Chemistry
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Bisphosphonates inhibit surface-mediated osteogenesis.

Ethan M Lotz1, Christoph H Lohmann2, Barbara D Boyan1,3

  • 1Department of Biomedical Engineering, College of Engineering, Virginia Commonwealth University, Richmond, Virginia, USA.

Journal of Biomedical Materials Research. Part A
|April 11, 2020
PubMed
Summary
This summary is machine-generated.

Bisphosphonates (BPs) reduce osteoblast production of key bone-forming markers, especially on microstructured surfaces. This impaired osteogenesis in vitro and in vivo suggests BPs may compromise osseointegration in clinical settings.

Keywords:
MG63bonemicrotopographyosteoblasttitanium

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Area of Science:

  • Biomaterials Science
  • Cell Biology
  • Orthopedic Research

Background:

  • Bisphosphonates (BPs) inhibit osteoclast activity, aiding osseointegration.
  • However, BPs may adversely impact osteoblasts, hindering peri-implant bone formation.

Purpose of the Study:

  • To investigate the effects of BPs on surface-mediated osteogenesis in osteoblasts.
  • To assess if BP exposure compromises the osteogenic response to microstructured titanium surfaces.

Main Methods:

  • MG63 cells and rat calvarial osteoblasts (rOBs) were cultured on smooth and microrough titanium disks or tissue culture polystyrene.
  • Cells were treated with varying concentrations of alendronate, zoledronate, or ibandronate.
  • Osteogenic markers in cell culture media were quantified using ELISAs; in vivo studies involved rat treatment with ibandronate.

Main Results:

  • BP treatment significantly reduced osteogenic markers (osteocalcin, OPG, BMP-2, etc.) in MG63 cells.
  • The inhibitory effect of BPs was more pronounced on microrough surfaces compared to smooth surfaces.
  • In vivo administration of ibandronate negatively conditioned rat osteoblasts, reducing their osteogenic marker production.

Conclusions:

  • Bisphosphonate exposure impairs the osteogenic response of osteoblasts to microstructured implant surfaces.
  • The negative effects of BPs on osteogenesis persist in vivo and in vitro.
  • Clinical use of BPs may potentially compromise osseointegration due to these detrimental effects on osteoblast function.