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Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up
Published on: March 26, 2018
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Acute lymphoblastic leukemia clonal distribution between bone marrow and peripheral blood
Carol Fries1, Diana G Adlowitz2, Janice M Spence2
1Department of Pediatrics, Division of Pediatric Hematology/Oncology, University of Rochester, Rochester, New York.
Pediatric Blood & Cancer
|April 12, 2020
Summary
Peripheral blood often misses key genetic details of B-acute lymphoblastic leukemia (B-ALL). Bone marrow analysis is crucial for understanding leukemia
Area of Science:
- Hematology
- Oncology
- Genetics
Background:
- Acute lymphoblastic leukemia (ALL) frequently presents with multiple subclones.
- Understanding clonal heterogeneity is vital for effective treatment strategies.
Purpose of the Study:
- To investigate if peripheral blood accurately reflects bone marrow clonal composition in B-ALL at diagnosis.
- To assess the impact of tissue sampling site on detecting leukemia subclones.
Main Methods:
- Utilized ultra-deep IGH sequencing on paired blood and bone marrow samples from 16 B-ALL patients.
- Analyzed clonal architecture and abundance across different tissue sites.
Main Results:
- Identified 28 distinct clones across 16 patients, with 5 clones exclusively found in bone marrow.
- Observed discrepancies in dominant clones between blood and marrow in 4 patients.
- Found that major bone marrow clones were often minimally detected in peripheral blood.
Conclusions:
- Peripheral blood samples frequently underrepresent the full genetic diversity of B-ALL.
- Tissue site selection significantly influences the detection of minor and dominant leukemia subclones.
- Bone marrow analysis is essential for a comprehensive understanding of B-ALL clonal heterogeneity.
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