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miR-206 family is important for mitochondrial and muscle function, but not essential for myogenesis in vitro

Roza K Przanowska1, Ewelina Sobierajska1, Zhangli Su1

  • 1Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, VA, USA.

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
|April 12, 2020
PubMed

Insights

The miR-206 family is not essential for skeletal muscle development but modulates differentiation. Triple knockout myoblasts show reduced mitochondrial function and adult mice have smaller myofibers and impaired performance.

Area of Science:

  • Molecular Biology
  • Muscle Physiology

Background:

  • MicroRNAs (miRNAs) like miR-206 and miR-1a are crucial for skeletal myogenesis.
  • Previous studies suggest miR-206 is vital for muscle regeneration in vivo.

Purpose of the Study:

  • To investigate the necessity of the miR-206 family in skeletal myogenesis.
  • To determine the role of miR-206, miR-1a-1, and miR-1a-2 in muscle differentiation and function.

Main Methods:

  • CRISPR/Cas9 gene editing was used to create a triple knockout (tKO) of miR-206, miR-1a-1, and miR-1a-2 in C2C12 myoblasts.
  • tKO mice were generated to assess in vivo effects on development and muscle physiology.

Main Results:

  • tKO myoblasts differentiated but exhibited diminished mitochondrial function.
  • tKO mice showed partial embryonic lethality and adult mice had smaller myofiber diameter, reduced physical performance, and increased PAX7+ satellite cells.
  • The absence of the miR-206 family did not prevent myogenesis but impacted its optimal execution.

Conclusions:

  • The miR-206 miRNA family is not absolutely essential for myogenesis but acts as a modulator for optimal skeletal muscle differentiation.
  • These findings highlight a nuanced role for specific miRNAs in complex developmental processes.
Keywords:
miR-1a-1miR-1a-2myogenesismyomiRsskeletal muscle differentiation

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