Differentiation of bone marrow stromal stem cells seeded on silk scaffold to mature oligodendrocyte using
Nourollah Rezaei1, Maryam Nazm Bojnordi1, Hatef Ghasemi Hamidabadi1
1Department of Anatomy & Cell Biology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran; Immunogenetic Research Center, Department of Anatomy & Cell Biology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
Abstract:
The differentiation of cultured Bone marrow stromal cells (BMSC) on silk scaffold into mature oligodendrocyte was done in the presence of cerebrospinal fluid (CSF). BMSC were isolated from Sprague-Dawley rats and were seeded on silk scaffold. The seeded cells were cultured in DMEM/F12 medium supplemented with CFS, basic fibroblast growth factor (bFGF), Retinoic acid (RA) and Epidermal growth factor (EGF). The glial differentiation was investigated using Real time-PCR and immunofluorescence techniques for specific glial markers: Oligo 2, NG2, PLP and MBP. Our dates showed that the differentiated cells expressed specific glial markers: Oligo 2, NG2, PLP and MBP. The specific mature oligodendrocyte genes were up regulated in cultured cells on silk scaffold in the presence of CSF. It is concluded that CSF leads to improve glial differentiation of seeded BMSC on silk scaffold using preparation of appropriate niche. This culture condition may be served as an efficient differentiation induction protocol for glial phenotype, with the perspective of therapeutic application in neuroregenerative medicine.
