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Clinical and Environmental Surveillance of Rotavirus Common Genotypes Showed High Prevalence of Common P Genotypes in

Waled M El-Senousy1, Amel S M Abu Senna2, Nabil A Mohsen3

  • 1Environmental Virology Lab., Water Pollution Research Department, Environmental Research Division and Food-Borne Viruses Group, Centre of Excellence for Advanced Sciences, National Research Centre (NRC), 33 El-Buhouth st., Dokki, P.O. 12622, Giza, Egypt. waledmorsy@hotmail.com.

Food and Environmental Virology
|April 13, 2020
PubMed

Insights

This study identified common human rotavirus group A G and P genotypes in Egyptian children and sewage. Findings aid future vaccine development by revealing prevalent strains and their genetic diversity.

Area of Science:

  • Virology
  • Public Health
  • Vaccinology

Background:

  • Human rotavirus group A (RVA) is a leading cause of severe diarrheal disease in children worldwide.
  • Current rotavirus vaccines, while effective, target specific G and P genotypes, necessitating ongoing surveillance for emerging strains.
  • Understanding the circulating RVA genotypes in a population is crucial for evaluating vaccine efficacy and guiding the development of new vaccines.

Purpose of the Study:

  • To compare the prevalence of common human rotavirus group A G and P genotypes in Egyptian stool specimens and raw sewage.
  • To inform future rotavirus vaccine development by identifying the most common circulating genotypes.
  • To assess the genetic similarity of local strains to existing vaccine strains.

Main Methods:

  • Nested reverse transcription-polymerase chain reaction (RT-PCR) was used to detect RVA group A in 1026 stool specimens.
  • Multiplex RT-PCR was employed to determine common G and P genotypes in positive clinical and raw sewage samples.
  • Phylogenetic analysis of the VP8 partial gene was conducted on selected isolates from clinical and sewage samples.

Main Results:

  • Human RVA group A was detected in 24.37% of pediatric stool specimens.
  • Common P genotypes were prevalent in 89.20% of positive clinical samples, while common G genotypes were found in 46.40%.
  • Raw sewage samples showed high detectability for P genotypes (87.50%) and moderate detectability for G genotypes (54.17%).

Conclusions:

  • The study identified specific common G and P genotypes of human rotavirus group A in Egypt.
  • The genetic diversity observed suggests potential limitations for current vaccine efficacy and highlights the need for updated vaccine formulations.
  • Findings provide valuable data for selecting target antigens for the development of more broadly protective rotavirus vaccines.

Keywords:
Common P and G genotypesDiarrheaHuman rotavirus group ANon-silent mutationSurveillance

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