Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Dec 24, 2025

Author Spotlight: Diatom Testing for Forensic Drowning Examination
04:20

Author Spotlight: Diatom Testing for Forensic Drowning Examination

Published on: November 10, 2023

2.7K

A DNA mini-barcode for marine macrophytes.

Alejandra Ortega1, Nathan R Geraldi2, Rubén Díaz-Rúa2

  • 1Biological and Environmental Science and Engineering Division (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia.

Molecular Ecology Resources
|April 13, 2020
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Oxygen Minimum Zone and Organic Carbon Structure Benthic Prokaryotic Communities and Metabolism in Warm Deep-Red Sea Sediments.

Microorganisms·2026
Same author

A global map of seagrass ecosystems.

Nature·2026
Same author

Polyp size predicts metabolic rates across diverse tropical coral species.

Communications biology·2026
Same author

Low Climate Benefit of Nordic Coastal Marshes: Site Conditions Outweigh Grazing Effects and Shape Trade-Offs Between Carbon Storage and Its Stability.

Global change biology·2026
Same author

Microbial Responses to Warming Reduce Deep Blue Carbon Storage.

Global change biology·2026
Same author

Stable bioreactor control reveals acidic pH-driven metabolic reprogramming and mitochondrial dysfunction in human lymphoblastoid cells.

Communications biology·2026
This summary is machine-generated.

Researchers identified the 18S gene as a universal DNA barcode for marine macrophytes, enabling accurate identification in environmental DNA (eDNA) from coastal sediments and supporting blue carbon research.

Area of Science:

  • Marine ecology
  • Molecular biology
  • Environmental DNA (eDNA) analysis

Background:

  • Marine macrophyte metabarcoding studies are limited by the lack of universal DNA barcodes and poor database representation.
  • Effective identification of marine flora is crucial for understanding coastal ecosystems and their role in carbon sequestration.

Purpose of the Study:

  • To identify a universal, short DNA barcode for marine macrophytes (seagrasses, mangroves, macroalgae).
  • To develop a DNA reference library for identifying marine macrophytes in environmental samples.
  • To assess the utility of the selected barcode for eDNA analysis in coastal sediments.

Main Methods:

  • Tested 18 primer pairs across six barcoding genes (rbcL, matK, trnL, ITS2, COI, 18S) on marine macrophyte tissue samples.
Keywords:
DNA barcodingeDNAenvironmental DNAmacroalgaemarine macrophytesmini-barcode

More Related Videos

A Standardized Procedure for Monitoring Harmful Algal Blooms in Chile by Metabarcoding Analysis
09:47

A Standardized Procedure for Monitoring Harmful Algal Blooms in Chile by Metabarcoding Analysis

Published on: August 26, 2021

5.9K
The Floating Lab: Standard Operational Procedure for Collecting and Filtering Seawater Samples from Operating Ferries for Environmental DNA Analysis
06:22

The Floating Lab: Standard Operational Procedure for Collecting and Filtering Seawater Samples from Operating Ferries for Environmental DNA Analysis

Published on: August 1, 2025

915

Related Experiment Videos

Last Updated: Dec 24, 2025

Author Spotlight: Diatom Testing for Forensic Drowning Examination
04:20

Author Spotlight: Diatom Testing for Forensic Drowning Examination

Published on: November 10, 2023

2.7K
A Standardized Procedure for Monitoring Harmful Algal Blooms in Chile by Metabarcoding Analysis
09:47

A Standardized Procedure for Monitoring Harmful Algal Blooms in Chile by Metabarcoding Analysis

Published on: August 26, 2021

5.9K
The Floating Lab: Standard Operational Procedure for Collecting and Filtering Seawater Samples from Operating Ferries for Environmental DNA Analysis
06:22

The Floating Lab: Standard Operational Procedure for Collecting and Filtering Seawater Samples from Operating Ferries for Environmental DNA Analysis

Published on: August 1, 2025

915
  • Utilized a phylogeny-based approach to assign taxonomy and construct an 18S DNA reference library.
  • Applied the 18S mini-barcode and reference library to identify marine macrophytes from coastal sediment eDNA.

    • Main Results:

    • The 18S gene demonstrated the highest universality, amplifying 95%-100% of marine macrophyte samples.
    • Tissue sequences were accurately identified to phylum (88%), order (76%), and genus (71%), with species-level identification at 23%.
    • Successfully recovered eDNA from 21 marine macrophytes in sediments, validating the 18S barcode's effectiveness for eDNA tracing.

    Conclusions:

    • The 18S gene serves as a robust universal barcode for marine macrophyte identification in both tissue and eDNA.
    • The developed 18S DNA reference library enhances the accuracy of macrophyte identification in environmental samples.
    • This approach facilitates ecological studies, including the assessment of blue carbon contributions and marine food web dynamics.