Antigens of Timothy and other grass pollen extracts identified by monoclonal antibodies

Using a series of monoclonal antibodies raised against pollen extract of timothy grass pollen (Phleum pratense) we identified individual (species-specific) and cross-reactive epitopes shared by different grass and tree pollens in EIA experiments. These epitopes are heterogenously distributed as detected by means of immunoblotting. Crossed radioimmunoelectrophoresis experiments revealed binding of monoclonal antibodies to major allergens of timothy grass pollen extract.