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Published on: July 31, 2014
Glutamine depletion in patients with Crimean-Congo hemorrhagic fever
Hüseyin Aydin1, Aynur Engin2, Sami Keleş3
1Department of Biochemistry, Sivas Cumhuriyet University School of Medicine, Sivas, Turkey.
Insights
This study reveals altered plasma amino acid levels in Crimean-Congo hemorrhagic fever (CCHF) patients, with lower glutamine and higher aspartate, glutamate, and gamma-aminobutyric acid. These findings offer insights into CCHF pathophysiology and potential supportive treatments.
Area of Science:
- Biochemistry
- Virology
- Clinical Medicine
Background:
- Crimean-Congo hemorrhagic fever (CCHF) is a severe viral illness with limited understanding of its metabolic alterations.
- Plasma amino acid profiles in CCHF patients remain largely uncharacterized, hindering insights into disease mechanisms.
Purpose of the Study:
- To investigate plasma amino acid levels in CCHF patients.
- To explore the association between specific amino acid levels and CCHF disease severity.
Main Methods:
- Liquid chromatography-mass spectrometry was employed to measure plasma concentrations of key amino acids: glutamine (Gln), glutamate (Glu), asparagine (Asn), aspartate (Asp), and gamma-aminobutyric acid (GABA).
- Comparison of amino acid levels between CCHF patients and healthy controls, and between fatal and non-fatal CCHF cases.
Main Results:
- CCHF patients exhibited significantly lower plasma Gln levels compared to controls.
- Elevated plasma levels of Asp, Glu, and GABA were observed in CCHF patients.
- Fatal CCHF cases showed increased Asn and decreased GABA levels.
Conclusions:
- This research is the first to report on plasma Gln, Glu, Asn, Asp, and GABA levels in CCHF.
- Altered amino acid profiles in CCHF may play a role in disease pathophysiology and immune response.
- Understanding these metabolic changes could inform supportive treatment strategies for CCHF.
Abstract:
Crimean-Congo hemorrhagic fever (CCHF) is a viral disease. There is not enough knowledge about plasma amino acid levels in CCHF. Therefore, we investigated plasma amino acid levels in patients with CCHF and the association between the levels of these amino acids and disease severity. The plasma amino acid levels (including glutamate [Glu], aspartate [Asp], glutamine [Gln], asparagine [Asn] and gamma-aminobutyric acid [GABA]) in CCHF patients and controls were measured by using liquid chromatography-mass spectrometry. Plasma levels of Gln were lower while Asp, Glu, and GABA levels were higher in patients. In fatal CCHF patients, we found the plasma level of Asn was increased whereas the plasma level of GABA was decreased. This study is the first in the literature to evaluate the plasma Gln, Glu, Asn, Asp, and GABA levels in CCHF patients. We found that the plasma Gln levels were significantly lower in CCHF patients while Asp, Glu, and GABA levels were elevated. Considering that these amino acids are important for immune cells, the plasma amino acid levels of CCHF patients may contribute to the understanding of the pathophysiology of disease and it can be important for supportive treatment of CCHF.
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