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Antimicrobial Colloidal Complexes of Lysozyme with Bio-Based Surface Active Ionic Liquids in Aqueous Medium
Gagandeep Singh1, Manvir Kaur1, Drishtant Singh2
1Department of Chemistry, UGC-Centre for Advance Studies - II, Guru Nanak Dev University, Amritsar, Punjab 143005, India.
The Journal of Physical Chemistry. B
|April 14, 2020
Summary
New bio-based surface-active ionic liquids (SAILs) form antimicrobial complexes with lysozyme (LYZ). These novel complexes enhance LYZ
Area of Science:
- Biochemistry and Materials Science
- Development of novel bio-based colloidal systems
Background:
- Surface-active ionic liquids (SAILs) offer tunable properties for various applications.
- Lysozyme (LYZ) is a protein with inherent antimicrobial properties.
- Combining biomolecules with synthetic compounds can lead to synergistic effects.
Purpose of the Study:
- To synthesize and characterize bio-based SAILs ([Cho][Sar] and [Cho][Doc]) for complexation with lysozyme (LYZ).
- To investigate the complexation behavior and structural alterations of LYZ upon interaction with SAILs.
- To develop novel antimicrobial colloidal complexes of SAILs and LYZ.
Main Methods:
- Synthesis of choline-based SAILs ([Cho]+) with lauryl sarcosinate ([Sar]-) or deoxycholate ([Doc]-) anions.
- Characterization using tensiometry, zeta-potential, turbidity, dynamic light scattering, fluorescence, circular dichroism (CD), and isothermal titration calorimetry (ITC).
- Computational simulations to determine SAIL binding sites on LYZ.
Main Results:
- Structurally different SAIL anions ([Sar]- vs. [Doc]-) exhibited contrasting complexation behaviors with LYZ.
- SAIL-LYZ complexation induced alterations in LYZ structure and interfacial properties.
- Lysozyme complexed with non-antimicrobial SAILs demonstrated enhanced antimicrobial activity.
Conclusions:
- Novel antimicrobial colloidal complexes of bio-based SAILs and LYZ were successfully developed.
- The study provides insights into designing bio-based SAILs for protein stabilization and biomedical applications.
- SAIL-LYZ complexes represent a promising platform for advanced antimicrobial formulations.
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