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Updated: Dec 24, 2025
Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays
Published on: June 28, 2024
A sensitive immunoassay for simultaneous detection of foodborne pathogens using MnO2 nanoflowers-assisted loading and
Li Xue1, Fengchun Huang1, Li Hao2
1Key Laboratory of Agricultural Information Acquisition Technology, Ministry of Agriculture and Rural Affairs, China Agricultural University, Beijing 100083, China.
Abstract:
In this study, a sensitive immunoassay using immunomagnetic nanobeads (MNBs), manganese dioxide nanoflowers (MnO2 NFs) and quantum dots (QDs) was developed for simultaneous detection of E. coli O157: H7 and Salmonella typhimurium. MnO2 NFs were synthesized, functionalized and incubated with QDs to obtain QDs@MnO2 nanocomposites, followed by modification with antibodies (pAbs) to obtain pAb-QDs@MnO2 nanocomposites (QM NCs). Target bacteria were first conjugated with MNBs and QM NCs to form MNB-bacteria-QM complexes. Then, QDs were quickly released from the complexes using glutathione to reduce MnO2 to Mn2+. Finally, fluorescent intensity at characteristic wavelength was measured by optical detector to determine target bacteria. This immunoassay could simultaneously and quantitatively detect E. coli from 1.5 × 101 to 1.5 × 106 CFU/mL with detection limit of 15 CFU/mL and Salmonella from 4.0 × 101 to 4.0 × 106 CFU/mL with detection limit of 40 CFU/mL in 2 h. The mean recovery for both bacteria in spiked chicken samples was ∼96%.
