Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Cadmium Sulfide/Pyrene Sulfone Polymer Nanoassemblies for Enhanced Photocatalytic Hydrogen Evolution.

Chemphyschem : a European journal of chemical physics and physical chemistry·2026

Same author

Plasmonic Engineering of Gold Nanoparticles Coupled Pyrene-Sulfone Polymer for Enhanced Photocatalytic Hydrogen Evolution.

Small (Weinheim an der Bergstrasse, Germany)·2026

Same author

Benzophenone-Based Polymers and Covalent Organic Framework for Photocatalytic Molecular Oxygen Activation.

ACS applied materials & interfaces·2025

Same author

Mesoporous Silica-Encapsulated Gold Nanorods for Drug Delivery/Release and Two-Photon Excitation Fluorescence Imaging to Guide Synergistic Phototherapy and Chemotherapy.

ACS applied bio materials·2023

Same author

Probing Excitonic Rydberg States by Plasmon Enhanced Nonlinear Optical Spectroscopy in Monolayer WS<sub>2</sub> at Room Temperature.

ACS nano·2022

Same author

Growth of bilayer MoTe<sub>2</sub> single crystals with strong non-linear Hall effect.

Nature communications·2022

Search research articles

Related Experiment Video

Updated: Dec 24, 2025

Synthesis, Characterization, and Functionalization of Hybrid Au/CdS and Au/ZnS Core/Shell Nanoparticles

Synthesis, Characterization, and Functionalization of Hybrid Au/CdS and Au/ZnS Core/Shell Nanoparticles

Published on: March 2, 2016

Aggregation-Induced Plasmon Coupling-Enhanced One- and Two-Photon Excitation Fluorescence by Silver Nanoparticles.

Ding-Feng Zhang¹, Shuang Li^1,2, Qing-Hua Xu^2,3

¹Institute of Polymer Optoelectronic Materials and Devices, State Key Laboratory of Luminescent Materials and Devices, South China University of Technology, Guangzhou 510640, P. R. China.

Langmuir : the ACS Journal of Surfaces and Colloids

|April 15, 2020

Summary

Aggregation of silver nanoparticles (Ag NPs) enhances dye fluorescence through plasmon coupling. Smaller Ag NPs showed greater fluorescence enhancement compared to larger ones when starting from a quenched state, enabling sensitive detection.

More Related Videos

Preparation of Silver-Palladium Alloyed Nanoparticles for Plasmonic Catalysis under Visible-Light Illumination

Preparation of Silver-Palladium Alloyed Nanoparticles for Plasmonic Catalysis under Visible-Light Illumination

Published on: August 18, 2020

Colloidal Synthesis of Nanopatch Antennas for Applications in Plasmonics and Nanophotonics

Colloidal Synthesis of Nanopatch Antennas for Applications in Plasmonics and Nanophotonics

Published on: May 28, 2016

Related Experiment Videos

Last Updated: Dec 24, 2025

Synthesis, Characterization, and Functionalization of Hybrid Au/CdS and Au/ZnS Core/Shell Nanoparticles

Synthesis, Characterization, and Functionalization of Hybrid Au/CdS and Au/ZnS Core/Shell Nanoparticles

Published on: March 2, 2016

Preparation of Silver-Palladium Alloyed Nanoparticles for Plasmonic Catalysis under Visible-Light Illumination

Preparation of Silver-Palladium Alloyed Nanoparticles for Plasmonic Catalysis under Visible-Light Illumination

Published on: August 18, 2020

Colloidal Synthesis of Nanopatch Antennas for Applications in Plasmonics and Nanophotonics

Colloidal Synthesis of Nanopatch Antennas for Applications in Plasmonics and Nanophotonics

Published on: May 28, 2016

Area of Science:

Nanotechnology
Optical Spectroscopy
Materials Science

Background:

Metal nanoparticles (NPs) create intense local electrical fields via plasmon coupling, enhancing chromophore optical properties.
Dye fluorescence can be quenched when attached to metal NPs, offering potential for turn-on detection strategies.

Purpose of the Study:

To investigate aggregation-induced plasmon coupling-enhanced one-photon excitation (1PE) and two-photon excitation (2PE) fluorescence of dyes.
To evaluate the effect of silver nanoparticle (Ag NP) size on fluorescence enhancement.

Main Methods:

Utilized silver nanoparticles (Ag NPs) of three sizes (20, 36, and 48 nm).
Studied the fluorescence of Rhodamine B isothiocyanate (RiTC), a model dye, prequenched on Ag NPs and enhanced upon NP aggregation.
Compared fluorescence enhancement based on free dyes versus prequenched dyes.

Main Results:

Aggregates of larger Ag NPs yielded greater 1PE and 2PE fluorescence enhancement relative to free dyes.
Aggregates of smaller Ag NPs (20 nm) provided larger enhancement (8.0-fold for 1PE, 22.5-fold for 2PE) relative to prequenched dyes.
Achieved fluorescence enhancement significantly exceeding conventional turn-on probes, indicating potential for sensitive detection with low background.

Conclusions:

Aggregation-induced plasmon coupling in Ag NPs effectively enhances dye fluorescence beyond simple recovery.
The size of Ag NPs influences the magnitude and basis of fluorescence enhancement.
This approach offers a promising route for developing highly sensitive fluorescence turn-on detection systems.