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Related Experiment Video

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Rodent Arena Tracker (RAT): A Machine Vision Rodent Tracking Camera and Closed Loop Control System.

Jonathan Krynitsky1, Alex A Legaria2, Julia J Pai2

  • 1Signal Processing and Instrumentation Section, Office of Intramural Research, Center for Information Technology (CIT), National Institutes of Health, Bethesda, MD 20814.

Eneuro
|April 15, 2020
PubMed
Summary

We developed a low-cost, open-source rodent arena tracker (RAT) using machine vision. This pocket-sized device enables real-time video tracking for rodent research without a tethered computer, making it scalable and accessible.

Keywords:
machine visionmouserodenttrackingvideo

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Area of Science:

  • Ethology
  • Neuroscience
  • Biomedical Engineering

Background:

  • Video tracking is crucial for behavioral analysis in rodent research.
  • Existing systems can be expensive and require tethered computers.
  • There is a need for accessible, scalable, and self-contained tracking solutions.

Purpose of the Study:

  • To develop and validate a low-cost, open-source rodent tracking device.
  • To create a pocket-sized, autonomous machine vision-based position tracker.
  • To facilitate rodent behavior research in resource-limited settings.

Main Methods:

  • Utilized an OpenMV Cam M7 for real-time video processing at 15 Hz.
  • Developed the Rodent Arena Tracker (RAT) with onboard microSD storage and a real-time clock.
  • Integrated a programmable multi-function input/output pin for device control and data transmission.
  • Open-sourced design files, build instructions, and code for accessibility.

Main Results:

  • The RAT processes positional data in real-time, reducing storage and data handling needs.
  • Demonstrated capabilities through circadian activity monitoring, pellet dispenser integration, and optogenetic stimulation control.
  • The device is pocket-sized, autonomous, and costs approximately $120 to build.
  • Validation studies confirmed its utility in diverse behavioral neuroscience experiments.

Conclusions:

  • The Rodent Arena Tracker (RAT) offers a cost-effective, scalable, and autonomous solution for rodent video tracking.
  • Its open-source nature promotes accessibility and further development in research and education.
  • Real-time processing and integrated features simplify data acquisition and experimental control.