Related Experiment Video
Updated: Dec 24, 2025

A Strategy to Validate the Role of Callose-mediated Plasmodesmal Gating in the Tropic Response
Published on: April 17, 2016
Modulation of auxin formation by the cytosolic phenylalanine biosynthetic pathway
Joseph H Lynch1, Yichun Qian2,3, Longyun Guo1,4
1Department of Biochemistry, Purdue University, West Lafayette, IN, USA.
Abstract:
In plants, phenylalanine biosynthesis occurs via two compartmentally separated pathways. Overexpression of petunia chorismate mutase 2 (PhCM2), which catalyzes the committed step of the cytosolic pathway, increased flux in cytosolic phenylalanine biosynthesis, but paradoxically decreased the overall levels of phenylalanine and phenylalanine-derived volatiles. Concomitantly, the levels of auxins, including indole-3-acetic acid and its precursor indole-3-pyruvic acid, were elevated. Biochemical and genetic analyses revealed the existence of metabolic crosstalk between the cytosolic phenylalanine biosynthesis and tryptophan-dependent auxin biosynthesis mediated by an aminotransferase that uses a cytosolic phenylalanine biosynthetic pathway intermediate, phenylpyruvate, as an amino acceptor for auxin formation.
Related Concept Videos
Amino Acid Biosynthetic Pathways
Plant Hormones
Cell Signaling in Plants
C4 Pathway and CAM
C4 Pathway
The C4 pathway is used by plants such as...
Allosteric Proteins-ATCase
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis...
Biosynthesis of Nucleic Acids

