Streptomyces soli sp. nov., isolated from birch forest soil

A Gram-staining positive, aerobic, non-motile, rod-shaped bacterium, designated strain LAM7114^T, was isolated from soil sample collected from a birch forest in Xinjiang Uygur Autonomous Region, China. The optimal temperature and pH for the growth of strain LAM7114^T were 30 °C and 7.0, respectively. Strain LAM7114^T could grow in the presence of NaCl up to 10% (w/v). Comparative analysis of the 16S rRNA gene sequences revealed that LAM7114^T was closely related to the members of the family genus Streptomyces, with the highest similarity to Streptomyces urticae NEAU-PCY-1^T (98.3%) and Streptomyces fildesensis GW25-5^T (98.2%). The genomic G + C content was 70.0 mol%. The DNA-DNA hybridization values between strain LAM7114^T and S. urticae CCTCC AA 2017015^T, S. fildesensis CGMCC 4.5735^T were 32.5 ± 1.8% and 27.5 ± 2.6%, respectively. The cell wall contained LL-diaminopimelic acid as the diagnostic diamino acid in the peptidoglycan. The whole-cell hydrolysates included glucose and mannose. The major fatty acids were anteiso-C_15:0, iso-C_15:0 and iso-C_16:0. The predominant menaquinones were MK-9(H₆), MK-9(H₄) and MK-9(H₈). The main polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, three unidentified aminophospholipids, three unidentified phospholipids, and an unidentified aminolipid. Based on the phenotypic characteristics and genotypic analyses, we propose that strain LAM7114^T represents a novel species in the genus Streptomyces, for which the name Streptomyces soli sp. nov. is proposed. The type strain is LAM7114^T (= CGMCC 4.7581^T = JCM 32822^T).